Agrobacterium tumefaciens-mediated transient transformation of Arabidopsis thaliana leaves.
Identifieur interne : 000423 ( Main/Exploration ); précédent : 000422; suivant : 000424Agrobacterium tumefaciens-mediated transient transformation of Arabidopsis thaliana leaves.
Auteurs : Silvina Mangano [Argentine] ; Cintia Daniela Gonzalez ; Silvana PetruccelliSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2014.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Arabidopsis (croissance et développement), Arabidopsis (génétique), Arabidopsis (métabolisme), Feuilles de plante (croissance et développement), Feuilles de plante (génétique), Feuilles de plante (métabolisme), Génie génétique (méthodes), Microscopie confocale (MeSH), Microscopie de fluorescence (MeSH), Protéines luminescentes (biosynthèse), Protéines luminescentes (génétique), Techniques de culture (MeSH), Techniques de transfert de gènes (MeSH), Transformation génétique (MeSH), Végétaux génétiquement modifiés (croissance et développement), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme), Électroporation (MeSH).
- MESH :
- biosynthèse : Protéines luminescentes.
- croissance et développement : Arabidopsis, Feuilles de plante, Végétaux génétiquement modifiés.
- génétique : Agrobacterium tumefaciens, Arabidopsis, Feuilles de plante, Protéines luminescentes, Végétaux génétiquement modifiés.
- métabolisme : Arabidopsis, Feuilles de plante, Végétaux génétiquement modifiés.
- méthodes : Génie génétique.
- Microscopie confocale, Microscopie de fluorescence, Techniques de culture, Techniques de transfert de gènes, Transformation génétique, Électroporation.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Arabidopsis (genetics), Arabidopsis (growth & development), Arabidopsis (metabolism), Culture Techniques (MeSH), Electroporation (MeSH), Gene Transfer Techniques (MeSH), Genetic Engineering (methods), Luminescent Proteins (biosynthesis), Luminescent Proteins (genetics), Microscopy, Confocal (MeSH), Microscopy, Fluorescence (MeSH), Plant Leaves (genetics), Plant Leaves (growth & development), Plant Leaves (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (metabolism), Transformation, Genetic (MeSH).
- MESH :
- chemical , biosynthesis : Luminescent Proteins.
- genetics : Agrobacterium tumefaciens, Arabidopsis, Luminescent Proteins, Plant Leaves, Plants, Genetically Modified.
- growth & development : Arabidopsis, Plant Leaves, Plants, Genetically Modified.
- metabolism : Arabidopsis, Plant Leaves, Plants, Genetically Modified.
- methods : Genetic Engineering.
- Culture Techniques, Electroporation, Gene Transfer Techniques, Microscopy, Confocal, Microscopy, Fluorescence, Transformation, Genetic.
Abstract
Transient assays provide a convenient alternative to stable transformation. Compared to the generation of stably transformed plants, agroinfiltration is more rapid, and samples can be analyzed a few days after inoculation. Nevertheless, at difference of tobacco and other plant species, Arabidopsis thaliana remains recalcitrant to routine transient assays. In this chapter, we describe a transient expression assay using simple infiltration of intact Arabidopsis leaves with Agrobacterium tumefaciens carrying a plasmid expressing a reporter fluorescent protein. In this protocol, Agrobacterium aggressiveness was increased by a prolonged treatment in an induction medium deficient in nutrients and containing acetosyringone. Besides, Arabidopsis plants were cultivated in intermediate photoperiod (12 h light-12 h dark) to promote leaf growth.
DOI: 10.1007/978-1-62703-580-4_8
PubMed: 24057365
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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uniqKey="Gonzalez C" first="Cintia Daniela" last="Gonzalez">Cintia Daniela Gonzalez</name></author><author><name sortKey="Petruccelli, Silvana" sort="Petruccelli, Silvana" uniqKey="Petruccelli S" first="Silvana" last="Petruccelli">Silvana Petruccelli</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24057365</idno><idno type="pmid">24057365</idno><idno type="doi">10.1007/978-1-62703-580-4_8</idno><idno type="wicri:Area/Main/Corpus">000434</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000434</idno><idno type="wicri:Area/Main/Curation">000434</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000434</idno><idno type="wicri:Area/Main/Exploration">000434</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Agrobacterium tumefaciens-mediated transient transformation of Arabidopsis thaliana 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(MeSH)</term><term>Végétaux génétiquement modifiés (croissance et développement)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term><term>Électroporation (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Luminescent Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéines luminescentes</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Arabidopsis</term><term>Feuilles de plante</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Arabidopsis</term><term>Luminescent Proteins</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="growth & development" 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Compared to the generation of stably transformed plants, agroinfiltration is more rapid, and samples can be analyzed a few days after inoculation. Nevertheless, at difference of tobacco and other plant species, Arabidopsis thaliana remains recalcitrant to routine transient assays. In this chapter, we describe a transient expression assay using simple infiltration of intact Arabidopsis leaves with Agrobacterium tumefaciens carrying a plasmid expressing a reporter fluorescent protein. In this protocol, Agrobacterium aggressiveness was increased by a prolonged treatment in an induction medium deficient in nutrients and containing acetosyringone. Besides, Arabidopsis plants were cultivated in intermediate photoperiod (12 h light-12 h dark) to promote leaf growth. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24057365</PMID><DateCompleted><Year>2014</Year><Month>04</Month><Day>10</Day></DateCompleted><DateRevised><Year>2013</Year><Month>09</Month><Day>23</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1062</Volume><PubDate><Year>2014</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Agrobacterium tumefaciens-mediated transient transformation of Arabidopsis thaliana leaves.</ArticleTitle><Pagination><MedlinePgn>165-73</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-62703-580-4_8</ELocationID><Abstract><AbstractText>Transient assays provide a convenient alternative to stable transformation. Compared to the generation of stably transformed plants, agroinfiltration is more rapid, and samples can be analyzed a few days after inoculation. Nevertheless, at difference of tobacco and other plant species, Arabidopsis thaliana remains recalcitrant to routine transient assays. In this chapter, we describe a transient expression assay using simple infiltration of intact Arabidopsis leaves with Agrobacterium tumefaciens carrying a plasmid expressing a reporter fluorescent protein. In this protocol, Agrobacterium aggressiveness was increased by a prolonged treatment in an induction medium deficient in nutrients and containing acetosyringone. Besides, Arabidopsis plants were cultivated in intermediate photoperiod (12 h light-12 h dark) to promote leaf growth. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mangano</LastName><ForeName>Silvina</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA)-CCT-La Plata-CONICET, Universidad de La Plata, La Plata, Argentina.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gonzalez</LastName><ForeName>Cintia Daniela</ForeName><Initials>CD</Initials></Author><Author ValidYN="Y"><LastName>Petruccelli</LastName><ForeName>Silvana</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008164">Luminescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C413662">red fluorescent protein</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & 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Confocal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008856" MajorTopicYN="N">Microscopy, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>9</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>9</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>4</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24057365</ArticleId><ArticleId IdType="doi">10.1007/978-1-62703-580-4_8</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Argentine</li></country></list><tree><noCountry><name sortKey="Gonzalez, Cintia Daniela" sort="Gonzalez, Cintia Daniela" uniqKey="Gonzalez C" first="Cintia Daniela" last="Gonzalez">Cintia Daniela Gonzalez</name><name sortKey="Petruccelli, Silvana" sort="Petruccelli, Silvana" uniqKey="Petruccelli S" first="Silvana" last="Petruccelli">Silvana Petruccelli</name></noCountry><country name="Argentine"><noRegion><name sortKey="Mangano, Silvina" sort="Mangano, Silvina" uniqKey="Mangano S" first="Silvina" last="Mangano">Silvina Mangano</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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